SOLUTIONS

Aqueous solutions of poly hexamethylenebiguanide salt [stable]

Sterisept & infection control

Sterisept is a highly effective, fast-acting biocide and very safe used as a disinfectant for hospitals and pharmaceutical industries.

Sterisept used for disinfection of equipment, floors, and other hard surfaces.

SteriSept composition & Specification

SteriSept based on polyhexamethylene biguanide hydrochloride (PHMB)

an extraordinary form of biosurfactant, biocatalyst, and activator, which provides the following properties:

• Extensive toxicity studies suggest acceptable use risk to humans
• Used as eye drops, impregnated wet wipes, and skin creams and lotions, and wound care as a clean solution for eye lenses.
• Fast-acting bactericide, active at low concentrations against Gram-positive and Gram-negative vegetative bacteria. At higher concentrations effective against yeasts and fungi
• Non-specific mode of action with no known evidence of the development of organism resistance.
• Water-soluble; which means it is readily water rinsed so minimizing the risk of residues
• Therefore, it is the ideal solution for the treatment of all fungicidal and bacterial infections.
• A typical use levels have no smell, no taste and are non-staining
• Retains activity in the presence of organic matter, such as proteins, fat, and blood, so providing reliable performance in the presence of soils after poor cleaning
• Active in both soft and hard water
• Active pH: neutral 7 ± 0.1
• SteriSept is stable over a temperature range from 0 to 100 °C
• Readily formulated with non-ionic surfactants (e.g., alcohol ethoxylates) and sequestrates (e.g., EDTA, NTA)
• Non-corrosive, and compatible with common materials of construction at typical use levels
• In the recommended concentration, it can be used in the presence of human coworkers
• No effect on colors

Mechanism of Action

Polyhexamethylene biguanide (PHMB) is a polymeric cationic antimicrobial agent that has been deployed in consumer applications for over 20 years. While it shares many attributes with the simpler cationic agents, it has additional action mechanisms that render it unique amongst this generic class of antimicrobials.

The outermost surface of microbial cells universally carries a net negative charge, often stabilized by the presence of divalent cations such as Mg²⁺ and Ca²⁺

The microbial membranes are composed primarily of proteins, embedded within a lipid matrix and approximating to a bi-layer.

The proteins either fully traverse the two sides of the bilayer (integral proteins) or are peripheral and associate with one specific side. Many of these membrane proteins are required to maintain the structural integrity of the membrane.

PHMB, the active ingredients of SteriSept binds rapidly to the bilayer membrane and, in doing so, displaces the otherwise stabilizing presence of Ca²⁺ The interstitial hexamethylene groups of the polymer are hydrophobic yet sufficiently inflexible that they cannot interdigitate into the hydrophobic core of the cell membrane.

Therefore, a bridging of adjacent acidic-phospholipids is brought about by the interaction of the SteriSept active ingredients with the cell membrane. One additional feature of this interaction is that it will tend to become concentrated around any points of maximum charge density within the membrane normally carrier or integrated proteins. This leads to the loss of their function by inflicting changes in their boundary phospholipid environment leading to cellular leakage.

Viruses can be found everywhere. They can cause great havoc to humans, animals, and plants, from illness to death. Most virologists consider viruses non-living, as they do not meet all the criteria of the generally accepted definition of life process. A virus is not able to metabolize, grow, or reproduce on its own, but must take over a host cell that provides these functions without the aid of a host cell, and is therefore not considered “living.”

IONOSEPT® device completely ionizes SteriSept (H2O2) to OH- ions, which are ejected with a speed of 80m/s and reach everywhere, even the invisible areas.

OH- ions – from IONOSEPT® – penetrate the protein sheath, reaching the DNA and results in oxidation and decomposition of DNA.

the unique composition of 9% oxygenated water chemically fixed with silver particles (1 ppm) and a highly specialized electrical conductivity catalyst with ionization specifically for IonoSept device that transformed into ions (OH-), which maximizes its effectiveness against the destruction of microbes of all kinds and also Biofilm

• works to kill microbes in the air and sterilize the surfaces when deposited on them.
• the excess of these ions combines half an hour again after the treatment is finished
• Safe compounds are given as water and oxygen (H₂O + O₂).
• Completely safe, non-toxic, non-irritating to the skin or respiratory system, and has no cumulative effects.
• It has no smell or color and does not leave traces or stains on surfaces or clothes and at the same time is safe for the environment and food
• Not harmful to materials and minerals and does not cause alteration, damage, or corrosion to them.
• HydroSept is a revolutionary complete disinfection product developed in Germany.
• HydroSept is the ultimate and simple answer to destroying 99.9% of all harmful bacteria, fungus, and viruses.
• We have many case studies that prove the efficacy of the HydroSept technology against MRSA’s, VRE’s, C.Diff, H5N1, H1N1, Legionella, Aspergillus Niger and many other types of bacteria, fungus and viruses
• New-generation disinfection with the IonoSept/HydroSept concept
• The disinfectant product, HydroSept (and the other disinfectant products of the same line), is a liquid containing 6% of hydrogen peroxide (H₂O₂) in distilled water spiked with colloidal silver.
• The combination of speed and temperature during spraying ionizes the emitted particles and degrades the peroxide into highly oxidizing free radicals – very short-lived ‘superoxide’ ions – life span.
• As this degradation is fast, no volatile organic compounds are generated.
• In this non-wetting fog, the particle size (1: 7 µ on average) ensures slow and perfectly uniform sedimentation on every square centimeter of the treated premises with no humidity
• Hydrogen peroxide (H₂O₂) is a widely used biocide for disinfection, sterilization, and antisepsis.
• It is a clear, colorless liquid that is commercially available in a variety of concentrations ranging from 3 to 90%.
• H₂O₂ considered environmentally friendly because it can rapidly degrade into the innocuous products water and oxygen. Although pure solutions are generally stable, most contain stabilizers to prevent decomposition.
• H₂O₂ demonstrates broad-spectrum efficacy against viruses, bacteria, yeasts, and bacterial spores.

MicroSept M. antimicrobial products based on the active agent Poly-Hexa Methylene- Biguanide-Hydrochloride (PHMB), a broad-spectrum, fast-acting Microcide used in Medical Field,

Characteristics:

• Broad-spectrum Antimicrobial
• The fast bactericidal effect at low concentrations
• Non-corrosive
• Non-staining
• Odorless
• Non-volatile
• Features Biocide Activity
• Retained activity in the presence of organic matter.
• Active in both soft and hard water
• Effective in a wide range of pH (1-11)
• Low foaming ‘low surface tension.’
• Does not leave streak marks on surfaces
• Storage stable formulation
• Non-flammable & easy to handle.
• Heat stable
• Aqueous

Applications

MicroSept M. antimicrobials being primarily solid surface disinfectants are suitable
for a wide range of applications in various areas & fields including:

Hospitals, operating theatres, Pediatric & Maternity suites, intensive care units, operating table, Medical & Dental Laboratories & clinics. CSSD`s (Surgical & dental Instruments –Flexible & rigid endoscope. Pharmaceutical plants & sterile areas.

MicroSept activity:

• Broad-spectrum, fast-acting bactericide.
• No known development of organism resistance.
• Active against Gram ⁺ve and Gram ^–ve bacteria.
• Active against pathogenic organisms (e.g. E. coli 0157)
• Active against antibiotic-resistant bacteria (e.g. MRSA, VRE)
• Active against odor-causing bacteria.
• Active against amoeba, fungi & yeast.
• Retained activity in the presence of organic matter.
• Active against enveloped and naked DNA & RNA viruses including
• HIV
• Influenza A2, H5 N1
• Hepatitis A, B, C
• Herpes simplex
• Vaccinia

MicroSept M. has several mechanisms of action on micro-organisms. It means that bacterial resistance is very unlikely to occur.

Due to continuous exposure to food materials, food processing tools and equipment forma very rich media for the flourish of micro-organisms. Raw food materials itself is theperfect media to grow micro-organisms.

• Special formula based on PHMB (PolyHexaMethylene Biguanide).
• Fast-acting against all kinds of micro-organisms (bacteria, viruses, fungi, and spores).
• Retain its activity in organic matters.
• It has several mechanisms of action on micro-organisms; this means that bacterial

resistance is very unlikely to occur.

• Does not leave streaks or marks on surfaces.
• Harmless to humans, animals, and food, it’s also environment-friendly.
• Very safe: Non-toxic, non-carcinogenic, non-teratogenic, it doesn’t cause skin or

respiratory irritation, no accumulative traces.

• Fast bactericidal effect at low concentrations
• Long-lasting (stable) in different temperatures with the same efficiency.
• Odorless, colorless, non-staining.
• Aqueous, it dissolves in water.
• Chemically inactive (non-corrosive), i.e., it doesn’t react with materials or metals.

Range of Application:
Food Processing Plants
Dairies & Milking Parlors
Cheese Molding Firms
Poultry Hatcheries
Canned Food Pasteurizers
Beverage Bottling Plants And everywhere
Spectrum
MicroSept F has a broad spectrum against all types of micro-organisms, including:
Gram +ve Bacteria
Staphylococous aureus (MRSA)
Staphylococous aureus
Staphylococous pyogenes
Clostridium botulinum
Listeria monocytogenes
Bacillus subtilis
Bacillus cereus
Gram –ve Bacteria
Escherichia coli 0157:H7
Pseudomonas aeruginosa
Campylobacter jejuni
Legionella pneumophila
Proteus sp
Klebsiella sp
Salmonella sp
Fungi/Yeasts
Aspergillus niger
Candiada albicans
Viruses
Herpes simplex
Vaccinia
Hepatitis A, B & C
HIV

Four (4) different mechanisms of action:

• CATAIONIC ACTION

Poly-Hexamethylene Biguanide polymer – Microsept main active ingredient – possesses along a chain of +ve charged that bacterial attack surface and displaced Ca++ and Mg++, causing bacterial instability.

• STATIC ACTION

MicroSept +ve charged units attract functional protein enzymes (such as ATPase & Phospholipids), causing loss of their functions and subsequently cessation of the microorganism.

• CHEMO – MECHANICAL ACTION

Following the replacement of Ca++ and Mg++ in the membrane by Poly-Hexamethylene Biguanide Polymer, it binds with functional protein, vital enzymes & acidic phospholipids. It changes it energetically to favorable hexagonal phase leading to a total loss of permeability, so a loss of all cytoplasmic content causing the complete destruction of microorganisms.

• CRACKING ACTION

Microsept activator that posses a homogenous stable 4 –ve charges ‘EDTA like action’ acts by the fast attraction of icons from the microorganism coat (Bacteria, Viruses & Fungi) producing cracks. Such electric instability & cracks inactivate the microorganism immediately and give way to the Microsept main active ingredient ‘Poly-Hexamethylene Biguanide’ to destroy the microorganism’s internal structures.

Mechanism of action of MicroSept on viruses

1. The virus is mainly formed of DNA or RNA naked or coated.
2. The MicroSept main active ingredient “Poly-Hexamethylene Biguanide” PHMB has a very high affinity to bind with protein causing direct decomposition and destruction of both protein coat and DNA or RNA.